Mutants of Bacillus subtilis Blocked in Acetoin Reductase
نویسندگان
چکیده
منابع مشابه
Mutants of Bacillus subtilis
Enzyme activities of glycolysis and glyconeogenesis are present in spores of Bacillus subtilis, the rate-limiting step of glucose (GLC) metabolism being its phosphorylation. GLC allows initiation of germination in the presence of fructose (FRU) and asparagine (ASN), not because it is used via the EmbdenMeyerhof path, but because it is oxidized in the nonphosphorylated form via the spore-specifi...
متن کاملThe Bacillus subtilis ydjL (bdhA) gene encodes acetoin reductase/2,3-butanediol dehydrogenase.
Bacillus subtilis is capable of producing 2,3-butanediol from acetoin by fermentation, but to date, the gene encoding the enzyme responsible, acetoin reductase/2,3-butanediol dehydrogenase (AR/BDH), has remained unknown. A search of the B. subtilis genome database with the amino acid sequences of functional AR/BDHs from Saccharomyces cerevisiae and Bacillus cereus resulted in the identification...
متن کاملGrowth and sporulation of Bacillus subtilis mutants blocked in the pyruvate dehydrogenase complex.
Two "ACE" mutants of Bacillus subtilis which require acetate for growth on glucose minimal medium have been isolated. They do not grow with acetoin, 2,3-butanediol, fatty acids, isoleucine, lipoic acid, malic acid, pyruvic acid, succinic acid, thiamine, or valine, but respond somewhat to glutamate or citrate. The mutants lack the activity of the pyruvate dehydrogenase complex; they excrete pyru...
متن کاملBacillus subtilis CcdA-defective mutants are blocked in a late step of cytochrome c biogenesis.
Cytochromes of the c type contain covalently bound heme. In bacteria, they are located on the outside of the cytoplasmic membrane. Cytochrome c synthesis involves export of heme and apocytochrome across the cytoplasmic membrane followed by ligation of heme to the polypeptide. Using radioactive protoheme IX produced in Escherichia coli, we show that Bacillus subtilis can use heme from the growth...
متن کاملAlcohol-resistant sporulation mutants of Bacillus subtilis.
About 80% of Bacillus subtilis cells form spores when grown in nutrient broth. In medium containing various short-chain aliphatic alcohols, the frequency of sporulation was reduced to 0.5%. Mutants sporulated in the presence of alcohols at a frequency of 30 to 40%. Sporulation in the wild-type cells was sensitive to alcohol at the beginning of sporulation (stage zero). Sensitivity to alcohol in...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1973
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1973.tb03216.x